Interferon-β Pretreatment of Conventional and Plasmacytoid Human Dendritic Cells Enhances Their Activation by Influenza Virus
Figure 7
IFN priming allows cDCs to partially overcome inhibition by IFN antagonist protein of the influenza virus.
cDCs were either infected with PR8 virus (PR8) or PR8 virus lacking the NS1 protein (ΔNS1) or left non-infected (NI). NI and PR8 samples were also pretreated with IFN-β (50, 500, 5,000 units/ml) for 3 hours prior to the infection. Infection was stopped after 6 hours and mRNA expression patterns were determined for genes labeled. Mean of samples are depicted with error bars of the standard deviation of each sample. Data are representative of at least three independent experiments.