Host Cell Egress and Invasion Induce Marked Relocations of Glycolytic Enzymes in Toxoplasma gondii Tachyzoites
Figure 4
Different fixation conditions do not affect aldolase-1 distribution.
To ensure that the observed redistribution of aldolase-1 is not a fixation artifact we also compared the distribution of aldolase-1 and cytoplasmic YFP in extracellular (EC) and intracellular (IC) Toxoplasma fixed in methanol (MeOH) or 3% paraformaldehyde/0.01% glutaraldehyde (PFA/GAH). Merged images of the various proteins and the parasite nuclei are shown as well as DIC images. Bars = 2 µm.