Ameobal Pathogen Mimivirus Infects Macrophages through Phagocytosis
Figure 5
Dominant negative mutant of Eps15 does not inhibit APMV internalization.
RAW 264.7 macrophages (A), macrophages transiently transfected with GFP (B) and dominant-negative mutant of Eps15 (EΔ95/295) (C) were infected with APMV (50 PFU/cell) for 6 hours. Viral particles were visualized by immunofluorescence (middle panels). Viral particles were associated with macrophages transfected with EΔ95/295. (D) The number of APMV particles internalized was scored. The results, expressed as the percentage of APMV uptake relative to the control, are the mean±SD of 4 experiments. Scale bars represent 25 µm.