A Mycobacterial Enzyme Essential for Cell Division Synergizes with Resuscitation-Promoting Factor
Figure 3
M. tuberculosis ripA allele complements depletion.
(A) Predicted domains of M. tuberculosis RipA-like proteins and L. monocytogenes p60. The NLPC_P60 putative domain in p60 was previously shown to have endopeptidase activity against cell wall material and defines the family. (B) Gene neighbors and predicted operons of rip genes in M. tuberculosis. Operons are indicated with black arrows, rip genes are in black text, gene numbers are given above arrows and further annotation for some genes is provided below arrows. One gene on either side of the rip-operon is shown with gray arrows. (C) A ripA-smeg depletion strain containing the M. tuberculosis ripA allele, ripA-mtb, on an episomal construct with its native promoter grew like wildtype when depleted of ripA-smeg (no tetracycline), while a strain carrying an empty plasmid formed chains when ripA-smeg was depleted. The M. tuberculosis allele of the ripA paralogue, rv1478, was not able to complement M. smegmatis depleted of ripA. All strains grew like wildtype in the presence of inducer, though the RipA and RipA-like strains grew slightly shorter than empty vector in the presence of Tet. Membranes were visualized with TMA-DPH. Bacteria were visualized with 100× objective.