Salp15 Binding to DC-SIGN Inhibits Cytokine Expression by Impairing both Nucleosome Remodeling and mRNA Stabilization
Figure 5
Salp15 Inhibits DC-Induced T Cell Proliferation
(A and B) Salp15 impairs DCs in the induction of T cell proliferation as shown by a mixed lymphocyte reaction (MLR). After 18 h of stimulation with LPS in the presence of Salp15 (25 μg/ml), tick saliva (1:100), or controls, DCs were extensively washed and cultured with allogeneic PBLs (1 × 105) at different ratios (1:50 to 1:500) for 4 d. Lymphocyte proliferation was assessed by overnight incorporation of radioactive thymidine. Data are representative of results obtained from four donors. Bars represent the mean of triplicates within one experiment ± SE. Differences between the different conditions were analyzed by a two-sided one way ANOVA, implementing a one way analysis of variance with Dunnett multiple comparison test. A p-value < 0.05 was considered statistically significant and indicated with one asterisk (*).