Plasmodium falciparum Regulatory Subunit of cAMP-Dependent PKA and Anion Channel Conductance
Figure 4
pfpka-c and pfpka-r Transcription Profiles in Wild-Type and PfPKA-R Overexpressing Parasites
(A) Ethidium bromide staining and northern blot analysis on 3D7 asexual asynchronous parasites (lanes A) and Percoll purified 3D7 gametocytes (stages III and IV, 95% pure) (lanes G) for pfpka-c (left panel) and pfpka-r (right panel) transcripts.
(B) Analysis of the level of transcription profile of pfpka-c and (C) pfpka-r genes in transformed pHL-dhfr-luciferase (grey bars) and pHL-dhfr-pfpka-r (black bars) cell lines. Data were calculated from triplicated data from two different RNA extractions. All values are presented as relative copy number to the housekeeping gene seryl-tRNA synthetase (PF07_0073).