Organizational Changes of the Daughter Basal Complex during the Parasite Replication of Toxoplasma gondii
Figure 7
The Initial Construction of the TgMORN1 Ring Is Likely to Be Independent of the Structural Integrity of the Daughter Cortical Cytoskeleton (cf. Video S2)
A parasitophorous vacuole containing eight dividing parasites expressing EGFP-TgMORN1 (green) and mCherryFP-TgTubA1 (red) was treated with 2.5 μM oryzalin at time 0. No daughter cortical microtubules can be detected in these parasites, but the initiation (cf. Video S2, 33–48 min) and the construction (cf. Video S2, 60–120 min) of the basal complex are not affected. As previously reported [9,17], the spindle pole (arrowheads) fails to replicate in the presence of 2.5 μM oryzalin.
Insets: 2.5× magnification of regions indicated by the dotted frames.
All images are maximum intensity projections of deconvolved 3D stacks.