Structure of GrlR and the Implication of Its EDED Motif in Mediating the Regulation of Type III Secretion System in EHEC
Figure 5
General Secretion Profile of EHEC EDL933 Harboring pSA10-grlR and Expressing Wild-Type and Mutants of GrlR
Secreted proteins were concentrated from supernatants of bacterial culture grown in DMEM and resolved in 12% SDS-PAGE and stained with coomassie brilliant blue (upper panel). Western blot of EHEC total cell protein against 6His antibody (lower panel).