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Cross-Talk with Myeloid Accessory Cells Regulates Human Natural Killer Cell Interferon-γ Responses to Malaria

Figure 2

HLA-DR+ Accessory Cells Provide Activating Signals to NK Cells

PBMCs from malaria-naïve donors were depleted of different populations of accessory cells, and NK cell responses to iRBCs were analysed after 24 h of co-culture.

(A) Representative example of NK cell IFN-γ responses in undepleted PBMCs (top left), PBMCs depleted of all HLA-DR+ antigen-presenting cells (APC) (top right), CD14+ monocyte–depleted PBMCs (middle left), CD19+ B cell–depleted PBMCs (middle right), CD1c+ mDC–depleted PBMCs (bottom left), and BDCA-4+ pDC–depleted PBMCs (bottom right). FACS plots are gated on CD3 CD56+ lymphocytes. Percentages indicate the proportion of CD3 CD56+ NK cells that were positive for IFN-γ; data are based on the collection of 100,000 total events.

(B) NK cell IFN-γ production after depletion of different antigen-presenting cell populations. Percentage of IFN-γ NK cells relative to the response in undepleted PBMCs is shown; each spot represents data for a different donor. *, p < 0.05; ***, p < 0.0001; p–values are for paired t tests of the difference between depleted and undepleted cells:

Figure 2

doi: https://doi.org/10.1371/journal.ppat.0020118.g002