Envelope Exchange for the Generation of Live-Attenuated Arenavirus Vaccines
Figure 5
Attenuated Propagation of rLCMV/NJG but Not of rLCMV-ARM*
(A) BHK-21 cells (106 per M6 well) were infected with the indicated viruses at a multiplicity of infection of 0.01, and infectious virus in the supernatant was measured at the indicated time points. Symbols indicate the mean of three tissue culture wells (SD bars project into the symbol size).
(B) BHK-21 cells in M6 tissue culture wells were infected with LCMV-ARM or rLCMV-ARM* at multiplicity of infection of 0.01, and infectious virus in the supernatant was measured at the indicated time points. The symbols represent values from a single cell culture well. One representative experiment of two is shown.
(C) C57BL/6 mice infected i.v. with 105 PFU LCMV-ARM or rLCMV-ARM* were killed at the indicated time points, and virus titers in spleen were measured. Symbols represent individual mice. Analogous results were obtained from liver tissue (not shown). One representative experiment of two is shown.