Gene-Specific Countermeasures against Ebola Virus Based on Antisense Phosphorodiamidate Morpholino Oligomers
Figure 5
Treatment of Guinea Pigs with Antisense PMOs Increases Survival Following Lethal Ebola Virus Infection
Hartley guinea pigs (n = 6) weighing 400–500 g each were treated intraperitoneally with 10 mg each of VP24, VP35, and L PMO in PBS at −24 (triangle), +24 (circle), or +96 (square) h post challenge. Control guinea pigs were injected with PBS only (diamond). The guinea pigs were infected subcutaneously with ~1,000 pfu of EBOV and monitored for illness for 21 d.
(A) The data are presented as percent survival for each group.
(B) Viral titers in the serum of guinea pigs at 7 d post challenge (dpc). The data are presented as the titers of individual guinea pigs treated with PMOs at −24, +24, or +96 h post challenge or PBS only and the mean viral titer for each group (horizontal line). Animals which survived are represented by open symbols and those that succumbed to disease are represented by filled symbols.