Aspergillus fumigatus Triggers Inflammatory Responses by Stage-Specific β-Glucan Display
Figure 4
Dectin-1 Is Recruited to AMØ Phagosomes Containing Swollen Conidia with Surface-Exposed β-Glucans
(A–H) AMØs were harvested from C57BL/6 mice 45 min after intratracheal instillation of 107 heat-killed swollen conidia (A–D) or heat-killed resting conidia (E–H), processed at 4 °C, and immunostained for ingested conidial β-glucans with mAb 744, followed by a FITC-coupled anti-mouse IgM (B and F) and for Dectin-1 (C and G) with goat anti-Dectin polyclonal antibodies, followed by Alexa Fluor 594-coupled anti-goat IgG. AMØs were examined by DIC microscopy (A and E) and epifluorescence confocal microscopy (B–D and F–H).
(D and H) Merged images of β-glucan and Dectin-1 immunofluorescence.
Scale bar = 10 μm.