YvqE and CovRS of Group A Streptococcus Play a Pivotal Role in Viability and Phenotypic Adaptations to Multiple Environmental Stresses
Fig 5
Survival of GAS after exposure to stress conditions.
SSI-1 and JRS4 wild-type strains, and their corresponding mutant strains from overnight cultures were inoculated into fresh THY containing various stress-generating agents. An aliquot from each bacterial culture was used for the determination of the number of cfu/ml by colony counting on THY agar plates. The results were normalized to the OD600 at time zero, and percent survival at the indicated time was calculated. All results were shown as the mean with standard deviation of 3 independent experiments performed in triplicate. Asterisks indicate statistically significant differences compared to the results for the wild-type strain. *P < 0.05. Sharps indicate statistically significant differences between ΔyvqEC or ΔcovRS strain, and ΔyvqECΔcovRS strain: #P < 0.05, as determined by t-test.