A Therapeutic Uricase with Reduced Immunogenicity Risk and Improved Development Properties
Fig 5
Enzymatic activity comparison of RGD and SGD containing uricases.
Substrate (UA) depletion assays were performed to assess uricase activity. The rate (specific activity) of UA oxidation was calculated based on a linear decrease in absorbance. Solid lines represent Michaelis-Menten kinetic fits performed in Prism GraphPad. Both RGD and SGD containing Arthrobacter globiformis uricases appear to have comparable enzymatic activity.