Identification of a Highly Conserved Hypothetical Protein TON_0340 as a Probable Manganese-Dependent Phosphatase
Fig 1
Structural features of TON_0340 monomer.
(a) Overall structure. On the ribbon drawing (left), the secondary structural elements are numbered in the order of the appearance in the primary structure. The surface representation (right) highlights the cavity containing the bound Mn2+ ions (spheres). (b) Zn2+ ions interacting with a cluster of six acidic residues. The detailed interactions are shown in stereoviews with and without the final 2Fo-Fc electron density maps (1.0 σ). The bound zinc ions, (Zn1, Zn2, Zn3) are shown as orange spheres, and water molecules as red spheres.(c) Structural superposition of TON_0340 and the catalytic core of RecJ (PDB entry: 1IR6). The Cα traces are shown. The superposed α-helices and β-strands in TON_0340 are labeled. The bound Mn2+ ions, two in TON_0340 and one in RecJ, are shown in spheres.