Hyperthermia Influences the Effects of Sodium Channel Blocking Drugs in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Fig 4
Hyperthermia diminished the effect of flecainide and lidocaine on the late INa.
The late INa was recorded by a depolarizing pulse from the holding potential of -100 mV to -45 mV with the duration of 400 ms. The area under the current curve was measured between 50 ms and 350 ms of the depolarizing pulse to illustrate the quantity of the late INa. Mean values of the percent inhibition of late INa in the presence of flecainide (A), lidocaine (B), ajmaline (C) and ranolazine (D) at 36°C and 40°C as well as 36°C plus 20 μM TTX are shown by the bar graphs. Representative traces of late INa inhibited by flecainide and lidocaine at 36°C and 40°C are also shown. Values given are mean ± SEM. n, number of cells. ns, P>0.05.