A Study of Differential Expression of Testicular Genes in Various Reproductive Phases of Hemidactylus flaviviridis (Wall Lizard) to Derive Their Association with Onset of Spermatogenesis and Its Relevance to Mammals
Fig 4
Comparative analysis of mRNA expression of selected genes in testis of wall lizards and mice.
Shows level of mRNA expression of Hk1 (A& I), Nme5 (B& J), Akap4 (C& K), Arih1 (D &L), Rassf7 (E & M), Tubb4b (F & N), Bco2 (G & O) and Hmgb1 (H & P) in three reproductive phases of wall lizard and three developmental stages of mice testis respectively. Normalization of gene expression was done with reference gene Gapdh for wall lizard and Ppia for mouse. Correlation between relative fold change in genes expression and testis weight in wall lizard and mice for genesHk1 (Q), Nme5 (R), akap4 (S), Arih1 (T), Rassf7 (U) and Tubb4b (V). Bco2 (W), and Hmgb1 (X) were analyzed. A solid or broken line in scatter plots denotes linear regression. Open circle with dotted line represents mice and closed circle with solid line represents wall lizard relative gene expression with respect to testicular wt. in percentage. Rg-T, Rc-T, Ac-T are testes from regressed, recrudescent, and active phase of wall lizard. 5d-T, 20d-T, and 60d-T are testes from 5 days, 20 days and 60 days old mice. *p<0.05, **p<0.01 and ***p<0.001 represents the comparison of Rg-T with Rc-T and Ac-T in wall lizards and 5d-T with 20d-T and 60d-T in mice. #p<0.05, ##p<0.01 and ###p<0.001 represents the comparison of Rc-T with Ac-T in wall lizards and 20d-T with 60d-T in mice.