2-Deoxyglucose Reverses the Promoting Effect of Insulin on Colorectal Cancer Cells In Vitro
Fig 8
ATP content analysis and western blots of AMPK, p62 and LC3.
(A) The ATP levels following the treatment of 2DG and/or insulin(20μU/ml) for 24h in colorectal cancer cells. ATP levels were normalized to untreated control sample. Data are mean ± SD of 3 independent experiments. #, significant differences compared with controls (P < 0.05). * P < 0.05. (B)Western blot analysis of Phospho-AMPKα (Thr172) representing active AMPK, AMPKα representing total AMPK, p62, LC3A/B. GAPDH was used as a loading control.