Extremely Low-Frequency Electromagnetic Fields Promote In Vitro Neuronal Differentiation and Neurite Outgrowth of Embryonic Neural Stem Cells via Up-Regulating TRPC1
Fig 6
Effects of ELF-EMF exposure on bHLH genes expression.
eNSCs were cultured in differentiation medium and exposed to ELF-EMF for 3 days. The mRNA expression of pro-neuronal genes (A) and Hes genes (B) was analyzed by real-time PCR. The mRNA expression of both NeuroD and Ngn1 was increased after ELF-EMF exposure. ** p < 0.01 vs. sham groups. (C) Representative western blotting bands of the NeuroD and Ngn1 protein expression. Full-length blots are presented in S4A and S4B Fig. (D, E) Statistical results of western blotting results. * p < 0.01 vs. sham groups. (F) Representative images of NeuroD and Ngn1 staining. For all experiments, the data are from five independent experiments and are presented as the mean ± SEM.