Differentiation of Apical Bud Cells in a Newly Developed Apical Bud Transplantation Model Using GFP Transgenic Mice as Donor
Fig 4
Green fluorescence and podoplanin expression in the apical end of the mandibular incisor.
Figures are the area of (b) in Fig 2 here at medium magnification. (A) In the section stained with hematoxylin and eosin, an apical bud (asterisk), pre-ameloblasts (arrowhead), dental pulp cells (DP), odontoblasts (OB), and ameloblasts (AB) are observed. Boxed areas (a) and (b) are magnified in Figs 5 and 6, respectively. (B) Green fluorescence is observed in the apical bud (asterisk), pre-ameloblasts (arrowhead), ameloblasts (AB), odontoblasts (OB), and dental pulp cells (DP). Green fluorescence is found in the capillaries of the dental pulp (arrows) as well. (C) Podoplanin expression is observed in apical bud (asterisk), pre-ameloblasts (arrowhead), and odontoblasts (OB). Immunoreaction with anti-podoplanin is weaker in ameloblasts (AB) than in pre-ameloblasts (arrowhead), and is stronger in odontoblasts (OB) than in pre-odontoblasts (outlined arrowhead). (D) Merged image of B, C, and blue fluorescence of DAPI’s. Scale bars: 100 μm.