Differentiation of Apical Bud Cells in a Newly Developed Apical Bud Transplantation Model Using GFP Transgenic Mice as Donor
Fig 1
Green fluorescence expression in 1-week-old C57BL/6-Tg (CAG-EGFP) mouse mandibular incisors.
(A) Mandibular incisors of the C57BL/6-Tg (CAG-EGFP) mouse (green mouse, top) and the C57BL/6 mouse (wild type, bottom) in phase contrast image. Incisors from the green mice showed identical shapes, sizes, and development as those from the wild type mice. The graft tissue was separated at the yellow dotted line. (B) Fluorescent image of the same teeth as A. The green fluorescence is observed only in the mandibular incisor of the green mouse. (C) Sagittal section stained with hematoxylin and eosin of the green mouse mandibular incisor. Asterisk indicates the apical bud. The graft tissue was separated at the yellow dotted line. (D) Fluorescent image of the same section as C. Green fluorescence is observed throughout the section with different intensities. Scale bars: 200 μm in A and B; 100 μm in C and D.