Identification of an Alternative Splicing Product of the Otx2 Gene Expressed in the Neural Retina and Retinal Pigmented Epithelial Cells
Fig 4
Synergistic activation of the tyrosinase promoter by OTX2 isoforms and MITF.
(A) Luciferase assays performed using mouse Tyr promoter construct -2226/+63 on HEK293 cells. (B) Luciferase assays performed using the mouse Tyr promoter construct on pig primary RPE cells. (C) Luciferase assays performed using mouse Tyr promoter construct on HEK293 cells using co-transfection with MITF. (D) Luciferase assays performed using mouse Tyr promoter construct on pig primary RPE cells using co-transfection with MITF. Each transfection was repeated three times with quadruplicates. Error bars represent Standard deviation of the mean. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001 (Two-way ANOVA Dunnett test).