Heterologous Expression Screens in Nicotiana benthamiana Identify a Candidate Effector of the Wheat Yellow Rust Pathogen that Associates with Processing Bodies
Fig 7
PST02549 and TaEDC4 co-accumulate in P-bodies.
Live-cell imaging of PST02549-GFP and TaEDC4-mCherry in N. benthamiana leaf cells. Images show a single optical section of 0.8 μm. The white asterisk indicates a pavement cell expressing only the TaEDC4-mCherry fusion, in which no large P-body was detected. Proteins were transiently expressed in N. benthamiana leaf cells by agroinfiltration. Live-cell imaging was performed with a laser-scanning confocal microscope with a sequential scanning mode two days after infiltration. GFP and the chlorophyll were excited at 488 nm; the mCherry was excited at 561 nm. GFP (green), mCherry (red), and chlorophyll (blue) fluorescence were collected at 505–525 nm, 580–620 nm and 680–700 nm, respectively. Black arrowheads indicate P-bodies. White arrowheads: nuclei.