A Mutation in Plant-Specific SWI2/SNF2-Like Chromatin-Remodeling Proteins, DRD1 and DDM1, Delays Leaf Senescence in Arabidopsis thaliana
Fig 7
Expression of transcriptional gene silencing markers and histone methyltransferase/acetyltransferase genes in the drd1-6 mutant.
(A) Expression analysis using transcriptional gene silencing markers 180-bp centromeric repeats (CEN) and (B) transcriptionally silent information (TSI) of WT and the drd1-6 mutant during DIS. Relative RNA levels were measured by qRT-PCR and the values are normalized to ACTIN2 expression. Data indicate the mean ± SD (n = 9) from three independent experiments. Bars with the same letter are not significantly different at P < 0.05 by Tukey’s honestly significant difference (HSD) test. (C) RT-PCR analysis in the WT and the drd1-6 mutant leaves at the indicated days. SDG8, set domain group 8; SDG27, set domain protein 27; and HAC1: histone acetyltransferase of the CBP family 1. ACTIN2 was used as control. Representative data from three or more independent experiments with similar results are shown.