Dominant Expression of DCLK1 in Human Pancreatic Cancer Stem Cells Accelerates Tumor Invasion and Metastasis
Fig 1
Sphere formation, migration, and invasion assay in Gdeg cell lines.
(A) Representative microscopic images of spheres are shown. Gdeghigh-KLM1 and Gdeghigh-BxPC3 cells formed complete spheres, but Gdeglow-KLM1 and Gdeglow-BxPC3 cells did not. Scale bar, 50 μm. (B) The number of spheres (>50 μm in diameter) observed in each well (n = 6). Data are the mean ± SD. **p <0.01 compared with Gdeghigh. The two-sided Student’s t-test was used to compare two independent groups. (C and D) Cell migration and invasion were analyzed with a double-chamber assay using Gdeghigh-KLM1, Gdeglow-KLM1, Gdeghigh-BxPC3, and Gdeglow-BxPC3 cells. Representative microscopic images are shown. Quantification of migration and invasion is presented as the mean ± SD for three independent experiments. **p <0.01 compared with Gdeghigh cells.