Rational Design of a Carrier Protein for the Production of Recombinant Toxic Peptides in Escherichia coli
Fig 4
Protein expression of first generation mutants.
(A) SDS-PAGE analysis of insoluble (lanes 1, 3) and soluble (lanes 2, 4) fractions after cell lysis of ONC(YY)-(P)GKY20 and ONC(EYEY)-(P)GKY20 fusion proteins, respectively. (B) SDS-PAGE analysis of soluble (lane 5) and insoluble (lane 6) fractions after cell lysis of ONC(EYEY)-H6-(P)GKY20 fusion protein. Lane M: marker (14.3 kDa).