Reduced Glucose Sensation Can Increase the Fitness of Saccharomyces cerevisiae Lacking Mitochondrial DNA
Fig 3
Overexpression of Pde2p can diminish the transcriptional response to mtDNA deletion.
(A) IDR target genes activated by mtDNA loss are attenuated upon Pde2p overexpression. Wild-type strain BY4743 transformed with vector pRS426, plasmid b89 (pRS426-PDE2), or plasmid M489 (pRS426-TIP41) was treated with EtBr for 24 hr to force mtDNA loss. Gene expression levels were determined by next-generation sequencing and normalized to BY4743 ρ+ cells harboring vector pRS426. Genes selected for analysis were activated more than three-fold in ρ0 cells expressing vector pRS426 over ρ+ cells expressing the same plasmid, were statistically significant upon comparison of these two conditions (q < 0.05), and were listed as IDR targets in [127] or [128]. (B) Genes activated by the PDR pathway in ρ0 cells are reduced in expression by Pde2p overexpression. Analysis was performed as in (A), except genes selected for analysis were identified as PDR pathway targets in [129] or [130]. (C) Genes activated by the RTG signaling pathway in cells lacking a mitochondrial genome are decreased in expression by Pde2p overproduction. Analysis was performed as in (A), with RTG pathway targets provided by [41]. (D) Arginine biosynthesis genes are upregulated upon mtDNA loss, but this response is reduced upon PKA inhibition. Analysis was performed as in (A), with arginine biosynthesis genes reported by [124]. (E) Two genes involved in aromatic amino acid breakdown and reduced in expression following mtDNA loss are recovered in expression when Pde2p is overexpressed. Analysis was performed as in (A). ARO9 and ARO10 were selected after a more than three-fold (q < 0.05) reduction in expression when comparing ρ0 cells to ρ+ cells containing vector pRS426. Quantitative expression data can be found in S1 Table. Data are visualized using [131].