Ultra-Deep Bisulfite Sequencing to Detect Specific DNA Methylation Patterns of Minor Cell Types in Heterogeneous Cell Populations: An Example of the Pituitary Tissue
Fig 4
Hypo-allele analysis of five T-DMRs of pituitary cell type-restricted genes.
(A) Gh1, Prl, Lhb, Tbx19, and Pit1 were analyzed using MiSeq sequencing. Horizontal lines indicate each sequenced read with methylation statuses of unmethylated (white) or methylated (black) CpGs. Sequenced reads above the dotted lines indicate Hypo-alleles. The numbers below the panels indicate CpG positions in bisulfite PCR products. (B) Summary of Hypo-allele (%) based on the MiSeq data of Fig 4A. (C) Expression levels of mRNAs of the five pituitary cell type-restricted genes and an internal control Gapdh gene in pituitary (Pi) and liver (Li).