Murine Embryonic Stem Cell Plasticity Is Regulated through Klf5 and Maintained by Metalloproteinase MMP1 and Hypoxia
Fig 5
MMP1 metalloproteinase partly mimics the LIF effect.
Cells were depleted from LIF for the indicated time and induced with 100 ng/mL of purified MMP1 as indicated. After 4 days, expression of the selected genes (A) Master genes, (B) Pluri genes and (C) Diff genes was analyzed by RT-qPCR. Graphs represent the average level of expression and SEM as depicted in Fig 2. One sample t-test was performed for each condition versus the +LIF sample: *p-value<0.05; **p-value<0.01, ***p-value<0.001; if not stated: not significant.