Mulberry Transcription Factor MnDREB4A Confers Tolerance to Multiple Abiotic Stresses in Transgenic Tobacco
Fig 5
The differences between the transgenic line and WT tobacco under normal conditions.
(a) The OE1 leaves were smaller and greener than the WT leaves. (b) The OE1 roots were longer than the WT roots. (c) The OE1 plants showed lower rates of water loss at each time point than the WT after leaf detachment and incubation at 30°C and 40% relative humidity. The significantly different values between the WT and OE1 plants are indicated by asterisks (n = 3). A single asterisk indicates a significant difference at P < 0.05 and two asterisks indicates a significant difference at P < 0.01. (d) The leaf discs were cultured in 1/2MS nutrient solution for about a month. The discs from WT plants became yellow and senescence was more obvious than on the transgenic tobacco OE1 leaf discs. The top line is the leaf discs before culturing, and the bottom line is the leaf discs after 1 month’s culturing.