Adaptation in Toxic Environments: Arsenic Genomic Islands in the Bacterial Genus Thiomonas
Fig 5
Schematic diagram of ICE19 in CB2.
This figure shows one part of the arsenic island of CB2 (RGP19) that has the characteristics of an ICE (called ICE19) (A) Alignment of ICE19 of Thiomonas sp. CB2 with its cognate portion found in the strain 3As (ThGEI-O). The arsenic island RGP19 of CB2 is localized in a different genomic region that ThGEI-O in 3As. The ThGEI-O of 3As (upper portion of the figure) is localized in a gene encoding a 4Fe-4S ferredoxin which is intact in CB2. Direct repeat sequences are indicated. (B) Sequence comparison of the almost perfect direct repeat flanking the ICE19. attL: left DR; attR: right DR. (C) Schematic representation of the integrated and circular form of the ICE19 of CB2, which were detected by PCR. The sequence attI is identical to attR and attB identical to attL. The % of nucleotide identity is expressed along a grey scale. Figures were generated with Easyfig [50].