A Novel Bmal1 Mutant Mouse Reveals Essential Roles of the C-Terminal Domain on Circadian Rhythms
Fig 2
Representative double-plot actograms and locomotor activities of WT, Bmal1+/GTΔC, Bmal1GTΔC/GTΔC, Bmal1+/- and Bmal1-/-mice.
(A) The representative 40-day wheel-running actograms under 10 days of L:D = 12 h:12 h and 40 days under DD are shown. The light and dark phases are illustrated by bright fields and gray shadows within the figure, respectively. (B) FRPs of each genotype. The FRPs of WT and Bmal1+/- mice were calculated from the data of initial 10 days in DD. Those of Bmal1+/GTΔC mice were divided as two different periods. “First” indicates the initial 5 days when the mice were addressed in DD and “Last” indicates the interval of 5 days before losing their rhythms. (C) Effects of genotypes on total activity of wheel running activity. The wheel running data were collected and analyzed. One-way ANOVA along with Tukey’s post-tests was conducted for statistical evaluation. Asterisks indicate significant differences (***p<0.001) compared with WT mice. Data are represented as the mean ± S.E.M. (n = 4~8 per group).