High Throughput Sequencing of Entamoeba 27nt Small RNA Population Reveals Role in Permanent Gene Silencing But No Effect on Regulating Gene Expression Changes during Stage Conversion, Oxidative, or Heat Shock Stress
Fig 3
E. invadens small RNA length and nucleotide distribution.
After removal of duplicates and size selection to remove RNAs >15nt or <40nt, a profile of length distribution and percent of each nucleotide base at each position were calculated for each library. Note the strong peak at 27nt in length and the preference for G at the 5' end in both datasets. (A) Trophozoite library, and (B) Mature cyst (72h) library.