Quantitative Brightness Analysis of Fluorescence Intensity Fluctuations in E. Coli
Fig 9
Measured stoichiometry of proteins in E. coli cells.
MSQ-curves were fit to Eqs 14 and 22 to determine the average stoichiometry n for EGFP (triangles) and NTF2-EGFP (squares) as a function of the initial fluorescence intensity F0. The average stoichiometry of EGFP (gray dashed line) is 0.98 ± 0.10. The average stoichiometry of NTF2-EGFP (blue dashed line) is 1.94 ± 0.27. The top axis displays the initial protein concentration.