Long-Term Expansion in Platelet Lysate Increases Growth of Peripheral Blood-Derived Endothelial-Colony Forming Cells and Their Growth Factor-Induced Sprouting Capacity
Fig 4
Comparison of proliferation-related features in PB-ECFCs during long-term cell expansion.
During long-term expansion of PB-ECFCs in PL-EGM, a set of experiments at different CPDLs (white bar 6CPDL, grey bar 18CPDL, black bar 31CPDL) were performed to investigate proliferation rate, telomere length and expression of the progenitor-cell marker CD34. (A) Comparison of population doubling time (PDT). (B): Telomere (T) and single copy gene (S) were amplified by quantitative real-time PCR, with the T/S ratio proportional to telomere length at different stages of cells’ age. (C): Flow cytometry data of percentage of cells positive for CD34. Results represent the mean ± SEM of 3 independent experiments each performed with different donor at indicated CPDL. Comparison between each CPDL was performed by one-way ANOVA with Bonferroni post hoc test (*p<0.05).