Increased Thymic Cell Turnover under Boron Stress May Bypass TLR3/4 Pathway in African Ostrich
Fig 7
Expression of TLR3 and TLR4 in ostrich thymus and bursa of fabricius.
(A) The quantification of TLR3 and TLR4 mRNA in ostrich thymus and bursa of fabricius at day 45 (n = 3) using real-time PCR. Vertical bars represent the mean±SEM, and bars with different letters mean significantly different (P < 0.05). (B) In ostrich thymus, TLR4-positive staining was mainly found in medulla and cortico-medullary junction (E), and a few in cortex (D). (C) In ostrich bursa of fabricius, TLR4-positive staining was observed in some mucosal epithelium (G) and medulla (F). The black rectangle in panels B and C is enlarged in right bottom of those panels. Co, cortex; Me, medulla; Mu, mucosal epithelium. Bars, 100 μm (B and C), 50 μm (D—F).