Invariant Natural Killer T Cells Play a Role in Chemotaxis, Complement Activation and Mucus Production in a Mouse Model of Airway Hyperreactivity and Inflammation
Fig 1
A-GalCer induces AHR, neutrophil influx and inflammation in lungs of WT mice.
A. Direct lung resistance (RL) was measured in WT mice after 24 hours after i.n. administration of vehicle or α-GalCer (αGC) using the ELAN system. B. Macrophage and neutrophil cell distributions were calculated (20x magnification) from cytospinned and MGG-stained BALF samples. C. Vehicle (upper figure) and α-GalCer—treated (lower figure), paraffin embedded lung sections were H&E stained, and D. Corresponding cryosections were stained with anti-CD3, anti-CD4 or with anti-CD8 mAbs to identify the number and localization of different T cell subsets. *P, 0.05; **P, 0.01; ***P, 0.001. n = 8 mice/group.