Identification of the Lipodepsipeptide MDN-0066, a Novel Inhibitor of VHL/HIF Pathway Produced by a New Pseudomonas Species
Fig 1
The automated workflow for the RCC4VA/VHL assay treated with natural extracts allows for the standardization of the process. Cells were seeded automatically and incubated overnight. The compounds or natural extracts from bacteria or fungi were transferred from mother plates to assay plates using a robotic workstation. After 24 hours of incubation at 37°C in medium containing the extracts or pure compounds cells were washed, fixed, and stained in a fully automated manner using a robotic workstation. For automated microscopy we used BD Pathway 855 High Content Bioimager.