Extremely Stable Soluble High Molecular Mass Multi-Protein Complex with DNase Activity in Human Placental Tissue
Figure 3
Gel filtration of the SPC proteins on a Sepharose 4B column (A) or Superdex 200 (B) corresponding to one placenta donor after the SPC treatment with buffer D with or without DTT (A and B): (—), absorbance at 280 nm (A280).
Fig. C shows gel filtration of the SPC on Superdex 200 after its previous proteins separation using Centricon membranes retaining molecules with MMs ≥100 kDa (bold line), with MMs 50–100 kDa (most bold line; the protein fraction passing-through membrane ≥100 kDa and then retained on membrane ≥50 kDa), and with MMs >10–30 kDa (thin line; the protein fraction passing-through membrane ≥30 and then proteins <10 kDa was partially removed from this fraction using membrane passing-through proteins with MMs <10 kDa). For details, see Materials and methods.