Intestinal Cell Kinase Is a Novel Participant in Intestinal Cell Signaling Responses to Protein Malnutrition
Figure 5
ICK deficiency in HCT-8 cells induced apoptosis via the caspase-dependent mechanism.
(A) HCT-8 cells expressing either the ICK-specific shRNA (shICK) or the control shRNA (shCTL) were assessed for the number of apoptotic cells using Annexin V staining. The relative fold change of Annexin V stain-positive cells was shown as mean ± SE, n = 4, **P<0.01. Similar results were obtained from two independent experiments. (B) Equal amount of total proteins from cell extracts was used on Western blot against antibodies recognizing key components in the caspase pathway as indicated. Western blot signals were quantified using densitometry and shown as the fold change after normalization.