Transcriptomic Analysis of Tail Regeneration in the Lizard Anolis carolinensis Reveals Activation of Conserved Vertebrate Developmental and Repair Mechanisms
Figure 5
Histological and RNA-Seq analysis of proliferation in the 25 dpa regenerating tail.
(A-E) MCM2 immunohistochemistry of the 25 dpa regenerating tail (brown nuclei), counterstained with hematoxylin (blue nuclei). (A) MCM2 is expressed throughout the regenerating tail, indicating a lack of a single proliferative zone. The condensing cartilage tube (B), ependymal core (C), developing muscles near the proximal base (D) and tip (E) of the regenerating tail are shown. (F) A heatmap showing gene expression of proliferative markers in the regenerating tail, the embryos, and satellite cells. DE genes along the regenerating tail axis are denoted with an asterisk. Composites: A. Scale bars in red: 200 µm (A) and 20 µm (B-E).