The Major Cow Milk Allergen Bos d 5 Manipulates T-Helper Cells Depending on Its Load with Siderophore-Bound Iron
Figure 2
Apo-, but not holo-Bos d 5 promotes CD4 cells.
PBMCs were treated with PMA alone or in the presence of apo- or holo-Bos d 5. (A) Living cells were gated on forward and side scatter, before gating on CD3 positive cells were performed. CD3 gated cells were then analyzed for their CD4 and CD8 expression.Representative pictograms of CD3 gated PBMCs plotted for CD4 and CD8 (B) Percentage of CD3+CD4+ cells. (C) Percentage of CD3+CD8+ cells. Statistical analyses were conducted with repeated measures ANOVA following Newman-Keuls Multiple Comparison test. **P<0.01, ****P<0.0001, ns not significant.