DP-b99 Modulates Matrix Metalloproteinase Activity and Neuronal Plasticity
Figure 4
DP-b99 prevents MMP-9 effect on dendritic spine morphology and F-actin arrangement.
A - Representative dendrites of GFP-transfected hippocampal neurons showing morphological changes in spines after indicated stimulations. Lower panels show the same dendrites following staining with Alexa Fluor 568 coupled to phalloidin (F-actin). Note that 1 h incubation of the cultures with MMP-9 (400 ng/ml) promotes dendritic spine elongation, whereas 1 h pretreatment with DP-b99 (0.12 µM) prevents the formation of these filopodia-like structures. Neither GM6001 nor DP-b99 on their own evoke spine elongation. B - Relative changes in spine length following 1 h stimulation with MMP-9 with or without DP-b99 or GM6001 pretreatment (n = 3, mean ± SE; *** - p<0.001). C - Fluorescence intensity of Alexa Fluor 568 coupled to phalloidin, calculated within spines vs. dendritic shaft (n = 3, mean ± SE; *** - p<0.001).