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The Phytochrome B/Phytochrome C Heterodimer Is Necessary for Phytochrome C-Mediated Responses in Rice Seedlings

Figure 5

phyC is biologically active in PHYB/aabb and PHYB(C364A)/aabb transgenic lines.

A. Diagram of PHYB and mutant PHYB(C364A) constructs introduced into phyB mutants. B. Immunoblot analyses of phyB and phyC levels in the dark-grown seedlings of PHYB/Aabb and PHYB(C364A)/Aabb transgenic lines. Protein extracts from 7-day-old etiolated seedlings of different transgenic lines of PHYB(C364A)/Aabb (#27, #69, #71, and #72) and PHYB/aabb (#7 and #11) were probed with anti-PHYC and anti-PHYB antibodies. WT and phyB were used as positive and negative controls, respectively. Each lane was loaded with 50 µg of protein extracts. C. FR inhibited coleoptile growth in PHYB/aabb transgenic lines. Mean coleoptile lengths were shown for the seedlings of WT, phyA, phyB, phyA phyB, and two lines of PHYB/aabb (#7-2- and #11-3-) grown under FR (15 µmol m−2 s−1, open bars) or in the dark (filled bars) for eight days. The mean ± SE obtained from at least 12 seedlings is plotted. D and E. FR (D) or R (E) inhibited coleoptile growth in the PHYB(C364A)/Aabb and PHYB(C364A)/aabb transgenic lines. Mean coleoptile lengths are shown for WT, phyA (only in D), phyB, phyA phyB, PHYB(C364A)/Aabb, and PHYB(C364A)/aabb seedlings grown under FR (15 µmol m−2 s−1, D), R (15 µmol m−2 s−1, E), or in the dark (filled bars) for eight days. Four independent lines (#27-6-, #69-2-, #71-1-, and #72-1-) of PHYB(C364A) were used, and results from different genetic backgrounds (Aabb or aabb) were depicted with separate bars. The mean ± SE obtained from at least 12 seedlings is plotted.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0097264.g005