Electroporation and Microinjection Successfully Deliver Single-Stranded and Duplex DNA into Live Cells as Detected by FRET Measurements
Figure 3
Fixed cell confocal microscopy images.
Left: Cy3 and Cy5 tagged DNA duplex (S1:S2) added to fixed/permeabilised cells and imaged using confocal microscopy. Images A/E represents the Cy3 channel; B/F the Cy5 channel; C/G the bright field channel and D/H an overlay of all the channels. Images A–D are excited with the 543 nm laser. Images E–H are excited with both the 543 and 633 nm lasers. Right: Intracellular fluorescence intensity from images A/B and E/F. Data are expressed as mean ± s.e.m from at least ten cells (p<0.001).