The Periplasmic Enzyme, AnsB, of Shigella flexneri Modulates Bacterial Adherence to Host Epithelial Cells
Figure 2
AnsB activity required for S. flexneri adherence to baby hamster kidney (BHK) cells.
Results of in vitro adherence assays for wild type S. flexneri serotype 3b (SFL1520), ΔansB (SFL2283) and complemented strain SFL2309 (SFL2283 + ansB). A: Bacterial adherence to baby hamster kidney cells plotted as percentage adherence, a ratio of the number of adherent and intracellular bacteria to the total number of bacterial cells in the infecting inoculum (y-axis). SFL2283 cells are significantly less adherent that SFL1520 and SFL2309 (p = 0.0003, One Way ANOVA). Results are the means of three independent blind repeats with standard errors. B–D: Results of coverslip adherence assay performed to compare the adherence of S. flexneri 3b wild type strain (SFL1520-B), ΔansB (SFL2283-C) and complement of ΔansB (SFL2309-D). E: A graph depicting the number of adherent bacteria counted per BHK cell under 1000× magnification (y-axis). The adherence of SFL2283 is significantly lower than SFL1520 and SFL2309 (p<0.0001, One Way ANOVA). Results are the mean of 10 cells across 10 fields with standard errors, counted at random in a blind experiment.