Activation of Mu Opioid Receptors Sensitizes Transient Receptor Potential Vanilloid Type 1 (TRPV1) via β-Arrestin-2-Mediated Cross-Talk
Figure 2
Treatment of primary sensory neurons with morphine or DAMGO, not herkinorin, decreases TRPV1 association with β-arrestin2.
TG neurons from rats were pretreated with morphine (1 μM), DAMGO (1 μM), herkinorin (10 μM), or vehicle (0.1% DMSO) for 15 min. Cells were rinsed and association of TRPV1 and β-arrestin2 was assessed via co-immunoprecipitation and Western blot (WB) analysis. A) Representative blot following immunoprecipitation (IP) for TRPV1 and probe (WB) for β-arrestin2 (β-Arr2) or TRPV1. B) Mean ± SEM from 3–5 independent experiments per treatment condition. *** p<0.001 by one-way ANOVA.