Correlative Scanning-Transmission Electron Microscopy Reveals that a Chimeric Flavivirus Is Released as Individual Particles in Secretory Vesicles
Figure 2
Analysis, by scanning electron microscopy (SEM), of Vero cells grown on microcarrier beads and infected with the chimeric YFV/DENV.
(A): At low magnification and before infection (day 0), Vero cells completely covered the microcarrier surface. (B): On day 0, analysis of these cells at high magnification showed them to have a smooth surface with protrusions. (C and D): On day 2 post-infection, several cells presented numerous chimeric viral particles at their surface (arrows). Scale bars : 100 µm in A ; 1 µm in B and C; 0.2 µm in D.