The Mechanisms Underlying α-Amanitin Resistance in Drosophila melanogaster: A Microarray Analysis
Figure 6
The qPCR results confirm the microarray data.
A) Relative expression distribution (Y-axis) of ten selected genes is shown as a ratio comparing Ama-KTT/M/2 and Canton-S (group 2 versus group 1). Each measurement contains 15 replicates (3 replicates for each of the five biological controls of groups 1 and 2). B) Gene expression differences between Ama-KTT/M/2 treated with α-amanitin and Ama-KTT/M/2 (group 3 versus group 2) are compared. Group 3 contributes to 18 data points (three replicates for each of the six biological controls), while group 2 contributes to 15 data points, as previously mentioned. All comparisons were normalized with two reference genes, Sucb and alpha-Tub84B. Ratios above one indicate that a gene is up-regulated in the comparison.