Metallic Nickel Nanoparticles May Exhibit Higher Carcinogenic Potential than Fine Particles in JB6 Cells
Figure 5
Effects of metallic nickel particles on colony formation of JB6 P+ cells in soft agar assay.
Metallic nickel nano- or fine particle-treated or untreated JB6 P+ cells (1×104) were incubated on soft agar medium for 21 days. TPA (20 ng/ml) was set as a positive control. (A) Images of colony formation of JB6 P+ cells in soft agar assay. (a) Control. (b) TPA (20 ng/ml). (c) Metallic nickel fine particles. (d) Metallic nickel nanoparticles. (B) Number of colonies/104 cells in soft agar assay. The cell colonies were scored by a computerized image analyzer. * P<0.05 versus untreated control.